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PRF1 Mouse mAb#64121

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Product Detail

Product NamePRF1 Mouse mAb

Clone No.MR4557

Host SpeciesMouse

ClonalityMonoclonal

IsotypeMouse IgG1

ApplicationsWB;IHC;FC

Species ReactivityHuman,Mouse

Immunogen DescPurified recombinant fragment of human PRF1 (AA: extra 148-372) expressed in E. Coli.

Target NamePRF1

Other NamesP1; PFP; HPLH2

Accession NoP14222

Uniprot P14222

Gene ID 5551

Calculated MW61.3kDa

FormulationPurified antibody in PBS with 0.05% sodium azide

StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Application Details
WB:1/500 - 1/2000
IHC:1/200 - 1/1000
FC:1/200 - 1/400
ELISA:1/10000
Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
All lanes : PRF1 Mouse mAb at 1/1k dilution
Lane 1 : Mouse liver lysates
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 61 kDa
Observed band size: 61 kDa
Exposure time: 4 seconds
Flow cytometric analysis of Jurkat cells using PRF1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using PRF1 mouse mAb with DAB staining.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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