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PRKAA2 Mouse mAb#64123

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Product Detail

Product NamePRKAA2 Mouse mAb

Host SpeciesMouse

ClonalityMonoclonal

IsotypeMouse IgG1

ApplicationsWB;IHC;IF;FC

Species ReactivityHuman,Mouse,Rat,Monkey,Rabbit

Immunogen DescPurified recombinant fragment of human PRKAA2 (AA: 453-552) expressed in E. Coli.

Target NamePRKAA2

Other NamesAMPK; AMPK2; PRKAA; AMPKa2

Accession NoP54646

Uniprot P54646

Gene ID 5563

Calculated MW62.3kDa

FormulationPurified antibody in PBS with 0.05% sodium azide

StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Application Details
WB:1/500 - 1/2000
IHC:1/100 - 1/500
ICC:1/50 - 1/500
FC:1/200 - 1/400
ELISA:1/10000
Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using PRKAA2 mouse mAb against HEK293 (1) and COS7 (2) cell lysate.
Western blot analysis using PRKAA2 mouse mAb against Mouse liver(1)PC-12(2)NIH/3T3(3)NRK(4)C2C12(5)C6(6)cell lysate.
Immunofluorescence analysis of NIH/3T3 cells using PRKAA2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Flow cytometric analysis of Jurkat cells using PRKAA2 mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues using PRKAA2 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using PRKAA2 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded Mouse kidney using PRKAA2 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded Rat kidney using PRKAA2 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded Rabbit kidney using PRKAA2 mouse mAb with DAB staining.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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