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RAD21 Mouse mAb#64163

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Product Detail

Product NameRAD21 Mouse mAb

Host SpeciesMouse

ClonalityMonoclonal

IsotypeMouse IgG1

ApplicationsWB;IHC;IF;FC

Species ReactivityHuman,Rat,Monkey

Immunogen DescPurified recombinant fragment of human RAD21 (AA: 287-403) expressed in E. Coli.

Target NameRAD21

Other NamesHR21; MCD1; NXP1; SCC1; CDLS4; hHR21; HRAD21

Accession NoO60216

Uniprot O60216

Gene ID 5885

Calculated MW71.7kDa

FormulationPurified antibody in PBS with 0.05% sodium azide

StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Application Details
WB:1/500 - 1/2000
IHC:1/200 - 1/1000
ICC:1/200 - 1/1000
FC:1/200 - 1/400
ELISA:1/10000
Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using RAD21 mouse mAb against Hela (1), HEK293 (2), K562 (3), C6 (4), *** (5), and COS7 (6) cell lysate.
Immunofluorescence analysis of Hela cells using RAD21 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of Hela cells using RAD21 mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human brain tissues using RAD21 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissues using RAD21 mouse mAb with DAB staining.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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