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SIGLEC8 Mouse mAb#64234

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Product Detail

Product NameSIGLEC8 Mouse mAb

Host SpeciesMouse

ClonalityMonoclonal

IsotypeMouse IgG2b

ApplicationsWB;IHC;FC

Species ReactivityHuman,Mouse,Rat

Immunogen DescPurified recombinant fragment of human SIGLEC8 (AA: extra 17-216) expressed in E. Coli.

Target NameSIGLEC8

Other NamesSAF2; SIGLEC-8; SIGLEC8L

Accession NoQ9NYZ4

Uniprot Q9NYZ4

Gene ID 27181

Calculated MW54kDa

FormulationPurified antibody in PBS with 0.05% sodium azide

StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Application Details
WB:1/500 - 1/2000
IHC:1/200 - 1/1000
FC:1/200 - 1/400
ELISA:1/10000
Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using SIGLEC8 mouse mAb against mouse Liver (1), rat Liver (2)tissues lysate, MCF-7 (3), and HT-29 (4) cell lysate.
Flow cytometric analysis of Jurkat cells using SIGLEC8 mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissues using SIGLEC8 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human rectum tissues using SIGLEC8 mouse mAb with DAB staining.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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