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SIRT1 Mouse mAb#64235

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Product Detail

Product NameSIRT1 Mouse mAb

Host SpeciesMouse

ClonalityMonoclonal

IsotypeMouse IgG1

ApplicationsWB;IHC;IF;FC

Species ReactivityHuman,Mouse,Rat,Rabbit,Monkey

Immunogen DescPurified recombinant fragment of human SIRT1 expressed in E. Coli.

Target NameSIRT1

Other NamesSIR2L1; SIRT1

Accession NoQ96EB6

Uniprot Q96EB6

Gene ID 23411

Calculated MW120kDa

FormulationPurified antibody in PBS with 0.05% sodium azide.

StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Application Details
WB:1/500 - 1/2000
IHC:1/200 - 1/1000
ICC:1/200 - 1/1000
FC:1/200 - 1/400
ELISA:1/10000
Western blot analysis using SIRT1 mouse mAb against MCF-7 (1), Jurkat (2), Hela (3), HEK293 (4) and A549 (5) cell lysate.
Western blot analysis using SIRT1 mouse mAb against L1210(1),F9,(2),Hela(3) cell lysate.
Immunofluorescence analysis of NTERA-2 cells using SIRT1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric analysis of K562 cells using SIRT1 mouse mAb (green) and negative control (purple).
Immunohistochemical analysis of paraffin-embedded human lung cancer tissues (left) and kidney cancer tissues (right) using SIRT1 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded Mouse testis(A)Mouse kidney(B) using SIRT1 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded Rat spleen(A)Rat liver(B) using SIRT1 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded Rabbit spleen(A)Rabbit liver(B) using SIRT1 mouse mAb with DAB staining.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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