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TNFSF11 Mouse mAb#64341

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Product Detail

Product NameTNFSF11 Mouse mAb

Host SpeciesMouse

ClonalityMonoclonal

IsotypeMouse IgG1

ApplicationsWB;IHC;IF;FC

Species ReactivityHuman,Mouse,Rat,Monkey,Rabbit

Immunogen DescPurified recombinant fragment of human TNFSF11 (AA: 74-308) expressed in E. Coli.

Target NameTNFSF11

Other NamesCD254; ODF; OPGL; sOdf; OPTB2; RANKL; TNLG6B; TRANCE; hRANKL2

Accession NoO14788

Uniprot O14788

Gene ID 8600

Calculated MW35.5kDa

FormulationPurified antibody in PBS with 0.05% sodium azide

StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Application Details
WB:1/500 - 1/2000
IHC:1/100 - 1/500
ICC:1/50 - 1/500
FC:1/200 - 1/400
ELISA:1/10000
Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using TNFSF11 mouse mAb against COS7 (1), Hela (2), U937 (3), HL-60 (4), Raji (5), Ramos (6), Jurkat (7), and SW480 (8) cell lysate.
Western blot analysis using TNFSF11 mouse mAb against PC-12(1)NIH/3T3(2)C2C12(3)C6(4)L1210(5)F9(6)COS-7(7)cell lysate.
Immunofluorescence analysis of Hela cells using TNFSF11 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of Hela cells using TNFSF11 mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using TNFSF11 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded Mouse brain(A) Mouse kidney(B) using TNFSF11 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded Rat brain(A) Rat kidney(B) using TNFSF11 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded Rabbit brain(A) Rabbit kidney(B) using TNFSF11 mouse mAb with DAB staining.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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