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USP7 Mouse mAb#64392

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Product Detail

Product NameUSP7 Mouse mAb

Host SpeciesMouse

ClonalityMonoclonal

IsotypeMouse IgG2b

ApplicationsWB;IHC;IF;FC

Species ReactivityHuman,Mouse,Rat,Monkey

Immunogen DescPurified recombinant fragment of human USP7 (AA: 1-208) expressed in E. Coli.

Target NameUSP7

Other NamesTEF1; HAUSP; HAFOUS

Accession NoQ93009

Uniprot Q93009

Gene ID 7874

Calculated MW128kDa

FormulationPurified antibody in PBS with 0.05% sodium azide

StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Application Details
WB:1/500 - 1/2000
IHC:1/200 - 1/1000
ICC:1/200 - 1/1000
FC:1/200 - 1/400
ELISA:1/10000
Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using USP7 mouse mAb against Hela (1), A431 (2), MCF-7 (3),Jurkat (4),K562 (5),HepG2 (6),A549 (7),HCT116 (8),HT-29 (9),SW480 (10),C6 (11),COS-7 (12),and NIH/3T3 (13) cell lysate.
Immunofluorescence analysis of Hela cells using mouse USP7 mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of Hela cells using USP7 mouse mAb (green) and negative control (red).
Flow cytometric analysis of HepG2 cells using USP7 mouse mAb (green) and negative control (red).
Flow cytometric analysis of Jurlat cells using USP7 mouse mAb (green) and negative control (red).
Flow cytometric analysis of K562 cells using USP7 mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissues using USP7 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human rectal cancer tissues using USP7 mouse mAb with DAB staining.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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