Product Detail
                                
                                                                        Product NameUSP7 Mouse mAb
                                                                                 Host SpeciesMouse
                                         ClonalityMonoclonal
                                        IsotypeMouse IgG2b
                                                                                ApplicationsWB;IHC;IF;FC
                                        Species ReactivityHuman,Mouse,Rat,Monkey
                                                                                                                         Immunogen DescPurified recombinant fragment of human USP7 (AA: 1-208) expressed in E. Coli.
                                         Target NameUSP7
                                                                                                                         Other NamesTEF1; HAUSP; HAFOUS
                                        Accession NoQ93009
                                        Uniprot
                                        Q93009
                                        
                                        Gene ID
                                        7874
                                        
                                        Calculated MW128kDa
                                                                                                                         FormulationPurified antibody in PBS with 0.05% sodium azide
                                        StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
                                                                                                                                                         
                                Application Details
                                WB:1/500 - 1/2000IHC:1/200 - 1/1000ICC:1/200 - 1/1000FC:1/200 - 1/400ELISA:1/10000
                             
                            
                                                                
                                    
                                    Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
                                 
                                                                
                                    
                                    Western blot analysis using USP7 mouse mAb against Hela (1), A431 (2), MCF-7 (3),Jurkat (4),K562 (5),HepG2 (6),A549 (7),HCT116 (8),HT-29 (9),SW480 (10),C6 (11),COS-7 (12),and NIH/3T3 (13) cell lysate.
                                 
                                                                
                                    
                                    Immunofluorescence analysis of Hela cells using mouse USP7 mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
                                 
                                                                
                                    
                                    Flow cytometric analysis of Hela cells using USP7 mouse mAb (green) and negative control (red).
                                 
                                                                
                                    
                                    Flow cytometric analysis of HepG2 cells using USP7 mouse mAb (green) and negative control (red).
                                 
                                                                
                                    
                                    Flow cytometric analysis of Jurlat cells using USP7 mouse mAb (green) and negative control (red).
                                 
                                                                
                                    
                                    Flow cytometric analysis of K562 cells using USP7 mouse mAb (green) and negative control (red).
                                 
                                                                
                                    
                                    Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissues using USP7 mouse mAb with DAB staining.
                                 
                                                                
                                    
                                    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissues using USP7 mouse mAb with DAB staining.