Product Detail
                                
                                                                        Product NameVASP Mouse mAb
                                                                                 Host SpeciesMouse
                                         ClonalityMonoclonal
                                        IsotypeMouse IgG1
                                                                                ApplicationsWB;IHC;IF;FC
                                        Species ReactivityHuman,Monkey
                                                                                                                         Immunogen DescPurified recombinant fragment of human VASP (AA: 1-380) expressed in E. Coli.
                                         Target NameVASP
                                                                                                                                                                Accession NoP50552
                                        Uniprot
                                        P50552
                                        
                                        Gene ID
                                        7408
                                        
                                        Calculated MW39.8kDa
                                                                                                                         FormulationPurified antibody in PBS with 0.05% sodium azide
                                        StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
                                                                                                                                                         
                                Application Details
                                WB:1/500 - 1/2000IHC:1/200 - 1/1000ICC:1/200 - 1/1000FC:1/200 - 1/400ELISA:1/10000
                             
                            
                                                                
                                    
                                    Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
                                 
                                                                
                                    
                                    Western blot analysis using VASP mouse mAb against THP-1 (1), HepG2 (2), Hela (3), HT-29 (4), MCF-7 (5), A549 (6), and COS-7 (7) cell lysate.
                                 
                                                                
                                    
                                    Immunofluorescence analysis of Hela cells using VASP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
                                 
                                                                
                                    
                                    Flow cytometric analysis of MOLT4 cells using VASP mouse mAb (green) and negative control (red).
                                 
                                                                
                                    
                                    Flow cytometric analysis of THP-1 cells using VASP mouse mAb (green) and negative control (red).
                                 
                                                                
                                    
                                    Flow cytometric analysis of HL-60 cells using VASP mouse mAb (green) and negative control (red).
                                 
                                                                
                                    
                                    Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using VASP mouse mAb with DAB staining.
                                 
                                                                
                                    
                                    Immunohistochemical analysis of paraffin-embedded human liver cancer tissues using VASP mouse mAb with DAB staining.
                                 
                                                                
                                    
                                    Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissues using VASP mouse mAb with DAB staining.