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WT1 Mouse mAb#64414

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Product Detail

Product NameWT1 Mouse mAb

Host SpeciesMouse

ClonalityMonoclonal

IsotypeMouse IgG1

ApplicationsWB;FC

Species ReactivityHuman,Monkey

Immunogen DescPurified recombinant fragment of human WT1 (AA: 1-181) expressed in E. Coli.

Target NameWT1

Other NamesGUD; AWT1; WAGR; WT33; NPHS4; WIT-2

Accession NoP19544

Uniprot P19544

Gene ID 7490

Calculated MW49.2kDa

FormulationPurified antibody in PBS with 0.05% sodium azide

StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Application Details
WB:1/500 - 1/2000
FC:1/200-1/400
ELISA:1/10000
Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
Western blot analysis using WT1 mouse mAb against K562 (1), COS7 (2), SK-OV-3 (3), Hela (4), and PC-3 (5) cell lysate.
Flow cytometric analysis of Hela cells using WT1 mouse mAb (green) and negative control (red).

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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