Product Detail
                                
                                                                        Product NameWTAP Mouse mAb
                                                                                 Host SpeciesMouse
                                         ClonalityMonoclonal
                                        IsotypeMouse IgG1
                                                                                ApplicationsWB;IHC;IF;FC
                                        Species ReactivityHuman,Mouse,Rat,Rabbit,Monkey
                                                                                                                         Immunogen DescPurified recombinant fragment of human WTAP (AA: 91-201) expressed in E. Coli.
                                         Target NameWTAP
                                                                                                                         Other NamesPNAS-132;hFL(2)D;KIAA0105
                                        Accession NoQ15007
                                        Uniprot
                                        Q15007
                                        
                                        Gene ID
                                        9589
                                        
                                        Calculated MW44.2kDa
                                                                                                                         FormulationPurified antibody in PBS with 0.05% sodium azide
                                        StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
                                                                                                                                                         
                                Application Details
                                WB:1/500 - 1/2000IHC:1/100 - 1/500ICC:1/50 - 1/500FC:1/200 - 1/400ELISA:1/10000
                             
                            
                                                                
                                    
                                    Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
                                 
                                                                
                                    
                                    Western blot analysis using WTAP mouse mAb against MCF-7 (1), Hela (2), K562 (3), Hek293 (4), A549 (5), HepG2 (6), Jurkat (7), and Cos7 (8) cell lysate.
                                 
                                                                
                                    
                                    Western blot analysis using WTAP mouse mAb against PC-12(1)NIH/3T3(2) NRK(3) C2C12 (4) C6(5) L1210(6) F9(7) COS7(8) CHO3D10(9) cell lysate.
                                 
                                                                
                                    
                                    Immunofluorescence analysis of Hela cells using WTAP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
                                 
                                                                
                                    
                                    Flow cytometric analysis of A549 cells using WTAP mouse mAb (green) and negative control (red).
                                 
                                                                
                                    
                                    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using WTAP mouse mAb with DAB staining.
                                 
                                                                
                                    
                                    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissues using WTAP mouse mAb with DAB staining.
                                 
                                                                
                                    
                                    Immunohistochemical analysis of paraffin-embedded Mouse testicles(A) Mouse cerebellum(B) using WTAP mouse mAb with DAB staining.
                                 
                                                                
                                    
                                    Immunohistochemical analysis of paraffin-embedded Rat brain(A) Rat testicles(B) using WTAP mouse mAb with DAB staining.
                                 
                                                                
                                    
                                    Immunohistochemical analysis of paraffin-embedded Rabbit testicles(A) Rabbit cerebellum(b) using WTAP mouse mAb with DAB staining.