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WTAP Mouse mAb#64416

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Product Detail

Product NameWTAP Mouse mAb

Host SpeciesMouse

ClonalityMonoclonal

IsotypeMouse IgG1

ApplicationsWB;IHC;IF;FC

Species ReactivityHuman,Mouse,Rat,Rabbit,Monkey

Immunogen DescPurified recombinant fragment of human WTAP (AA: 91-201) expressed in E. Coli.

Target NameWTAP

Other NamesPNAS-132;hFL(2)D;KIAA0105

Accession NoQ15007

Uniprot Q15007

Gene ID 9589

Calculated MW44.2kDa

FormulationPurified antibody in PBS with 0.05% sodium azide

StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Application Details
WB:1/500 - 1/2000
IHC:1/100 - 1/500
ICC:1/50 - 1/500
FC:1/200 - 1/400
ELISA:1/10000
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western blot analysis using WTAP mouse mAb against MCF-7 (1), Hela (2), K562 (3), Hek293 (4), A549 (5), HepG2 (6), Jurkat (7), and Cos7 (8) cell lysate.
Western blot analysis using WTAP mouse mAb against PC-12(1)NIH/3T3(2) NRK(3) C2C12 (4) C6(5) L1210(6) F9(7) COS7(8) CHO3D10(9) cell lysate.
Immunofluorescence analysis of Hela cells using WTAP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of A549 cells using WTAP mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using WTAP mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissues using WTAP mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded Mouse testicles(A) Mouse cerebellum(B) using WTAP mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded Rat brain(A) Rat testicles(B) using WTAP mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded Rabbit testicles(A) Rabbit cerebellum(b) using WTAP mouse mAb with DAB staining.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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