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Location： Home > Mouse Monoclonal Antibodies > CDKN2A Mouse mAb

CDKN2A Mouse mAb#64434

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Product Detail

Product NameCDKN2A Mouse mAb

Clone No.MR4530

Host SpeciesMouse

ClonalityMonoclonal

IsotypeMouse IgG1

ApplicationsWB,IHC,FCM,ELISA

Species ReactivityHuman

Immunogen DescPurified recombinant fragment of human CDKN2A (AA: 1-156) expressed in E. Coli.

Target NameCDKN2A

Other NamesARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A

Uniprot P42771

Gene ID 1029

Calculated MW17kD

Sdspage MW17kDa

FormulationPurified antibody in PBS with 0.05% sodium azide

StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Application Details
WB 1:500-1:2000
IHC 1:200-1:1000
FCM 1:200-1:400
ELISA 1:10000
Formalin-fixed, paraffin-embedded human ovarian cancer tissues stained for CDKN2A using 64434 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human endometrial cancer tissues stained for CDKN2A using 64434 at 1/100 dilution in immunohistochemical analysis.
Flow Cytometry Calreticulin antibody (64434)
Flow cytometric analysis of Hela cells with CDKN2A Mouse mAb at 1/50 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody，red).
Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
All lanes : CDKN2A Mouse mAb at 1/1k dilution
Lane 1 : Hela whole cell lysates
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDa
Exposure time: 6 seconds
Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein.

If you have published an article using product 64434, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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