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GFP-Tag Mouse mAb#64435

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Product Detail

Product NameGFP-Tag Mouse mAb

Clone No.MR4531

Host SpeciesMouse

ClonalityMonoclonal

IsotypeMouse IgG2a

ApplicationsWB,IP,IF,IHC,FCM,ELISA

Species Reactivitytag

Immunogen DescPurified recombinant fragment of GFP expressed in E. Coli.

Target NameGFP-Tag

Uniprot P42212

Calculated MW27kD

FormulationPurified antibody in PBS with 0.05% sodium azide

StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Application Details
WB 1:500-1:1000
IF 1:100-1:200
IHC 1:200-1:1000
FCM 1:200-1:400
IP 1:20-1:50
ELISA 1:10000
Formalin-fixed, paraffin-embedded HCC827 cells stained for GFP-Tag using 64435 at 1/100 dilution in immunohistochemical analysis,untransfected (left) or transfected with GFP (right).
Immunocytochemistry/ Immunofluorescence GFP antibody (64435)

ICC/IF staining of GFP antibody in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 64435 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti mouse, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
Flow Cytometry Calreticulin antibody (64435)
Flow cytometric analysis of HCC827 cells with GFP-Tag Mouse mAb at 1/50 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody,blue).
Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+-activated photoprotein aequorin.

If you have published an article using product 64435, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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