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Recombinant Human Eukaryotic translation initiation factor 4E-binding protein 3(EIF4EBP3)#AP70265

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Product Detail

Product NameRecombinant Human Eukaryotic translation initiation factor 4E-binding protein 3(EIF4EBP3)

Host SpeciesE.coli

PurificationGreater than 90% as determined by SDS-PAGE.

Immunogen DescExpression Region:1-100aa
Sequence Info:Full Length

Accession NoO60516

Uniprot O60516

Gene ID 8637;

Calculated MW26.9 kDa

FormulationTris-based buffer50% glycerol

StorageThe shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20˚C,-80˚C. The shelf life of lyophilized form is 12 months at -20˚C,-80˚C.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4˚C for up to one week.

Tag InfoN-terminal 6xHis-SUMO-tagged

Application Details
Repressor of translation initiation that regulates EIF4E activity by preventing its assbly into the eIF4F complex: hypophosphorylated form competes with EIF4G1,EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1,EIF4G3 and EIF4E, leading to initiation of translation.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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