Product Detail
Product NameRecombinant Chicken Glyceraldehyde-3-phosphate dehydrogenase(GAPDH)
Host SpeciesE.coli
PurificationGreater than 90% as determined by SDS-PAGE.
Immunogen DescExpression Region:2-333aa
Sequence Info:Full Length
Other NamesPeptidyl-cysteine S-nitrosylase GAPDH (EC:2.6.99.-)
Accession NoP00356
Uniprot
P00356
Gene ID
374193;
Calculated MW39.6 kDa
FormulationTris-based buffer50% glycerol
StorageThe shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20˚C,-80˚C. The shelf life of lyophilized form is 12 months at -20˚C,-80˚C.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4˚C for up to one week.
Tag InfoN-terminal 6xHis-tagged
Application Details
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assbly of the cytoskeleton. Also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins .
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15-25 business day
