Product Detail
Product NameRecombinant Human Histone H3.3(H3F3A),partial
Host SpeciesE.coli
PurificationGreater than 90% as determined by SDS-PAGE.
Immunogen DescExpression Region:2-136aa
Sequence Info:Partial
Accession NoP84243
Uniprot
P84243
Gene ID
3020;3021;
Calculated MW31.2 kDa
FormulationTris-based buffer50% glycerol
StorageThe shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20˚C,-80˚C. The shelf life of lyophilized form is 12 months at -20˚C,-80˚C.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4˚C for up to one week.
Tag InfoN-terminal 6xHis-SUMO-tagged
Application Details
Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacent throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a tplate. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome rodeling.
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8-15 business day
