Product Detail
Product NameRecombinant Mouse E3 ubiquitin-protein ligase Mdm2(Mdm2)
Host SpeciesE.coli
PurificationGreater than 90% as determined by SDS-PAGE.
Immunogen DescExpression Region:1-489aa
Sequence Info:Full Length
Other NamesDouble minute 2 proteinOncoprotein Mdm2p53-binding protein Mdm2
Accession NoP23804
Uniprot
P23804
Gene ID
17246;
Calculated MW58.6 kDa
FormulationTris-based buffer50% glycerol
StorageThe shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20˚C,-80˚C. The shelf life of lyophilized form is 12 months at -20˚C,-80˚C.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4˚C for up to one week.
Tag InfoN-terminal 6xHis-tagged
Application Details
E3 ubiquitin-protein ligase that mediates ubiquitination of p53,TP53, leading to its degradation by the proteasome. Inhibits p53,TP53- and p73,TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as a ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53,TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28,KAP1-MDM2-p53,TP53 complex involved in stabilizing p53,TP53. Also component of the TRIM28,KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways. Mediates ubiquitination and subsequent proteasome degradation of DYRK2 in nucleus. Ubiquitinates IGF1R and SNAI1 and promotes th to proteasomal degradation .
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