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Recombinant Escherichia coli Beta-lactamase TEM(bla) #AP71101

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Product NameRecombinant Escherichia coli Beta-lactamase TEM(bla)

Host SpeciesE.coli

PurificationGreater than 90% as determined by SDS-PAGE.

Immunogen DescExpression Region:24-286aa
Sequence Info:Full Length

Other NamesIRT-4PenicillinaseTEM-1TEM-16,CAZ-7TEM-2TEM-24,CAZ-6TEM-3TEM-4TEM-5TEM-6TEM-8,CAZ-2

Accession NoP62593

Uniprot P62593

Gene ID 10076131;10076142;13876868;13877052;13903673;13905334;13905363;13906404;13906709;13906924;13909533;13909568;14612524;17824300;17824435;18157686;20466965;20466993;20467118;20468340;20471961;20491414;20491639;20492529;20492626;20493584;2716540;3244915;37224

Calculated MW44.9 kDa

FormulationTris-based buffer50% glycerol

StorageThe shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20˚C,-80˚C. The shelf life of lyophilized form is 12 months at -20˚C,-80˚C.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4˚C for up to one week.

Tag InfoN-terminal 6xHis-SUMO-tagged

Application Details
T-type are the most prevalent beta-lactamases in enterobacteria; they hydrolyze the beta-lactam bond in susceptible beta-lactam antibiotics, thus conferring resistance to penicillins and cephalosporins. T-3 and T-4 are capable of hydrolyzing cefotaxime and ceftazidime. T-5 is capable of hydrolyzing ceftazidime. T-6 is capable of hydrolyzing ceftazidime and aztreonam. T-8,CAZ-2, T-16,CAZ-7 and T-24,CAZ-6 are markedly active against ceftazidime. IRT-4 shows resistance to beta-lactamase inhibitors.

If you have published an article using product AP71101, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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