Product Detail
Product NameRecombinant Moloney murine leukemia virus Gag polyprotein(gag) ,partial
Host SpeciesE.coli
PurificationGreater than 90% as determined by SDS-PAGE.
Immunogen DescExpression Region:216-478aa
Sequence Info:Partial
Other NamesCore polyprotein
Cleaved into the following 4 chains:
Matrix protein p15
Short name:
MA
RNA-binding phosphoprotein p12
Alternative name(s):
pp12
Capsid protein p30
Short name:
CA
Nucleocapsid protein p10
Short name:
NC-gag
Accession NoP03332
Uniprot
P03332
Gene ID
1491870;
Calculated MW46.6 kDa
FormulationTris-based buffer50% glycerol
StorageThe shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20˚C,-80˚C. The shelf life of lyophilized form is 12 months at -20˚C,-80˚C.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4˚C for up to one week.
Tag InfoN-terminal 6xHis-SUMO-tagged
Application Details
Gag polyprotein plays a role in budding and is processed by the viral protease during virion maturation outside the cell. During budding, it recruits, in a PPXY-dependent or independent manner, Nedd4-like ubiquitin ligases that conjugate ubiquitin molecules to Gag, or to Gag binding host factors. Interaction with HECT ubiquitin ligases probably link the viral protein to the host ESCRT pathway and facilitate release.
If you have published an article using product AP71111, please notify us so that we can cite your literature.
8-15 business day
