Product Detail
Product NameRecombinant Human papillomavirus type 16 Regulatory protein E2(E2)
Host SpeciesE.coli
PurificationGreater than 90% as determined by SDS-PAGE.
Immunogen DescExpression Region:1-365aa
Sequence Info:Full Length
Accession NoP03120
Uniprot
P03120
Gene ID
1489080;
Calculated MW68.8 kDa
FormulationTris-based buffer50% glycerol
StorageThe shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20˚C,-80˚C. The shelf life of lyophilized form is 12 months at -20˚C,-80˚C.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4˚C for up to one week.
Tag InfoN-terminal GST-tagged
Application Details
Plays an accessory role in initiation of DNA replication. A dimer of E2 interacts with a dimer of E1 in order to improve specificity of E1 DNA binding activity. Once the complex recognizes and binds DNA at specific sites, the E2 dimer is roved from DNA. E2 also regulates viral transcription through binding to the E2RE response elent (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory regions of the viral genome. Activates or represses transcription depending on E2RE's position with regards to proximal promoter elents including the TATA-box. Repression occurs by sterically hindering the assbly of the transcription initiation complex.
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