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Recombinant Escherichia coli Tetracycline repressor protein class B from transposon Tn10(tetR)#AP71195

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Product Detail

Product NameRecombinant Escherichia coli Tetracycline repressor protein class B from transposon Tn10(tetR)

Host SpeciesE.coli

PurificationGreater than 90% as determined by SDS-PAGE.

Immunogen DescExpression Region:1-207aa
Sequence Info:Full Length

Accession NoP04483

Uniprot P04483

Gene ID 31515962;4924774;

Calculated MW39.4 kDa

FormulationTris-based buffer50% glycerol

StorageThe shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20˚C,-80˚C. The shelf life of lyophilized form is 12 months at -20˚C,-80˚C.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4˚C for up to one week.

Tag InfoN-terminal 6xHis-SUMO-tagged

Application Details
TetR is the repressor of the tetracycline resistance elent; its N-terminal region forms a helix-turn-helix structure and binds DNA. Binding of tetracycline to TetR reduces the repressor affinity for the tetracycline resistance gene (tetA) promoter operator sites.

If you have published an article using product AP71195, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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