Product Detail
Product NameRecombinant Micrurus tener tener Kunitz-type neurotoxin MitTx-alpha
Host SpeciesE.coli
PurificationGreater than 90% as determined by SDS-PAGE.
Immunogen DescExpression Region:25-84aa
Sequence Info:Full Length
Accession NoG9I929
Uniprot
G9I929
Calculated MW23.1 kDa
FormulationTris-based buffer50% glycerol
StorageThe shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20˚C,-80˚C. The shelf life of lyophilized form is 12 months at -20˚C,-80˚C.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4˚C for up to one week.
Tag InfoN-terminal 6xHis-SUMO-tagged
Application Details
This heterodimeric toxin potently activates mouse acid-sensing ion channel ASIC1,ACCN2 expressed in Xenopus oocytes. Both alternatively spliced isoforms ASIC1a and ASIC1b are activated, with a higher potency for ASIC1a (EC(50)=9.4 nM) vs ASIC1b (EC(50)=23 nM). The ASIC3,ACCN3 subtype is also sensitive to the heterodimer, but with a lower potency (EC(50)=830 nM). On ASIC2a,ACCN1, the toxin shows a very weak activation, but produces a rarkable potentiation (>100-fold) of protons when the Extracellular domain pH drops below neutrality. The toxin interacts with the Extracellular domain region of the channel, since responses are only observed in the outside-out configuration. In vivo, the heterodimer elicits robust pain-related behavior in mice by activation of ASIC1,ACCN2 channels on capsaicin-sensitive nerve fibers.
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