Product Detail
Product NameRecombinant Bacillus subtilis Glycine oxidase(thiO)
Host SpeciesE.coli
PurificationGreater than 90% as determined by SDS-PAGE.
Immunogen DescExpression Region:1-369aa
Sequence Info:Full Length
Accession NoO31616
Uniprot
O31616
Gene ID
939377;
Calculated MW56.9 kDa
FormulationTris-based buffer50% glycerol
StorageThe shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20˚C,-80˚C. The shelf life of lyophilized form is 12 months at -20˚C,-80˚C.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4˚C for up to one week.
Tag InfoN-terminal 6xHis-SUMO-tagged
Application Details
Catalyzes the FAD-dependent oxidative deamination of various amines and D-amino acids to yield the corresponding alpha-keto acids, ammonia,amine, and hydrogen peroxide. Oxidizes sarcosine (N-methylglycine), N-ethylglycine and glycine. Can also oxidize the herbicide glyphosate (N-phosphonomethylglycine). Displays lower activities on D-alanine, D-valine, D-proline and D-methionine. Does not act on L-amino acids and other D-amino acids. Is essential for thiamine biosynthesis since the oxidation of glycine catalyzed by ThiO generates the glycine imine intermediate (dehydroglycine) required for the biosynthesis of the thiazole ring of thiamine pyrophosphate.
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15-25 business day
