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Recombinant Streptomyces achromogenes Modification methylase SacI(sacIM)#AP71223

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Product Detail

Product NameRecombinant Streptomyces achromogenes Modification methylase SacI(sacIM)

Host SpeciesE.coli

PurificationGreater than 90% as determined by SDS-PAGE.

Immunogen DescExpression Region:1-390aa
Sequence Info:Full Length

Other NamesCytosine-specific methyltransferase SacI

Accession NoO31073

Uniprot O31073

Calculated MW59.3 kDa

FormulationTris-based buffer50% glycerol

StorageThe shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20˚C,-80˚C. The shelf life of lyophilized form is 12 months at -20˚C,-80˚C.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4˚C for up to one week.

Tag InfoN-terminal 6xHis-SUMO-tagged

Application Details
This methylase recognizes the double-stranded sequence GAGCTC, causes specific methylation on C-4 on both strands, and protects the DNA from cleavage by the SacI endonuclease.

If you have published an article using product AP71223, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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