Product Detail
Product NameRecombinant Mouse Bis(5'-adenosyl)-triphosphatase(Fhit)
Host SpeciesE.coli
PurificationGreater than 90% as determined by SDS-PAGE.
Immunogen DescExpression Region:2-150aa
Sequence Info:Full Length
Other NamesAP3A hydrolase ;AP3AaseDiadenosine 5',5'''-P1,P3-triphosphate hydrolaseDinucleosidetriphosphataseFragile histidine triad protein
Accession NoO89106
Uniprot
O89106
Gene ID
14198;
Calculated MW21.1 kDa
FormulationTris-based buffer50% glycerol
StorageThe shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20˚C,-80˚C. The shelf life of lyophilized form is 12 months at -20˚C,-80˚C.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4˚C for up to one week.
Tag InfoN-terminal 6xHis-tagged
Application Details
Cleaves P(1)-P(3)-bis(5'-adenosyl) triphosphate (Ap3A) to yield AMP and ADP. Can also hydrolyze P(1)-P(4)-bis(5'-adenosyl) tetraphosphate (Ap4A), but has extrely low activity with ATP. Modulates transcriptional activation by CTNNB1 and thereby contributes to regulate the expression of genes essential for cell proliferation and survival, such as CCND1 and BIRC5. Plays a role in the induction of apoptosis via SRC and AKT1 signaling pathways. Inhibits MDM2-mediated proteasomal degradation of p53,TP53 and thereby plays a role in p53,TP53-mediated apoptosis. Induction of apoptosis depends on the ability of FHIT to bind P(1)-P(3)-bis(5'-adenosyl) triphosphate or related compounds, but does not require its catalytic activity . Functions as tumor suppressor.
If you have published an article using product AP71237, please notify us so that we can cite your literature.
15-25 business day
