Product Detail
Product NameCaspase 8 Antibody FITC Conjugated
Host SpeciesRabbit
ClonalityPolyclonal
IsotypeIgG
PurificationPurified by Protein A.
Applications IF
Species ReactivityHu Ms Rt
Immunogen DescKLH conjugated synthetic peptide aa 430-482 482 derived from mouse Caspase-8 subunit p10
Target NameCaspase 8
ConjugateFITC
Excitation Emission494nm 518nm
Other NamesMACH; Mch5; FLICE; CASP-8; Caspase-8; Casp8
Concentration1mg ml
Formulation0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
StorageShipped at 4˚C. Store at -20˚C for one year. Avoid repeated freeze/thaw cycles.
Application Details
IF=1:50-200
Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6 FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP1. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein.
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15 business days.
