Product Detail
Product NameHistone H3 Antibody PE Conjugated
Host SpeciesRabbit
ClonalityPolyclonal
IsotypeIgG
PurificationPurified by Protein A.
Applications Flow-Cyt ICC IF
Species ReactivityHu Ms Rt
Immunogen DescKLH conjugated synthetic peptide aa 90-136 136 derived from human Histone H3
Target NameHistone H3
ConjugatePE
Excitation Emission480,565nm 578nm
Other NamesH3F3; H3.3A; Histone H3.3; H3F3A; PP781
Accession NoSwiss-Prot#P84243
NCBI Gene ID3020
Uniprot
P84243
Gene ID
3020;3021;
Concentration1mg ml
Formulation0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
StorageShipped at 4˚C. Store at -20˚C for one year. Avoid repeated freeze/thaw cycles.
Application Details
Flow-Cyt=1:50-200 ICC=1:50-200 IF=1:50-200
Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
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15 business days.
