Product Detail
Product NameCyclin D1 Antibody FITC Conjugated
Host SpeciesRabbit
ClonalityPolyclonal
IsotypeIgG
PurificationPurified by Protein A.
ApplicationsFlow-Cyt IF
Species ReactivityHu Ms Rt
Immunogen DescKLH conjugated synthetic peptide aa 70-120 295 derived from mouse Cyclin D1
Target NameCyclin D1
ConjugateFITC
Excitation Emission494nm 518nm
Other NamescD1; CycD1; Cyl-1; PRAD1; bcl-1; AI32739; G1 S-specific cyclin-D1; Ccnd1
Accession NoSwiss-Prot#P25322
NCBI Gene ID12443
Uniprot
P25322
Gene ID
12443;
Concentration1mg ml
Formulation0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
StorageShipped at 4˚C. Store at -20˚C for one year. Avoid repeated freeze/thaw cycles.
Application Details
Flow-Cyt=1:50-500 IF=1:50-200
Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1) S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1 CDK4 CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner (By similarity).
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