Product Detail
Product NameCDK4 Antibody FITC Conjugated
Host SpeciesRabbit
ClonalityPolyclonal
IsotypeIgG
PurificationPurified by Protein A.
ApplicationsFlow-Cyt ICC IF
Species ReactivityHu Ms Rt
Immunogen DescKLH conjugated synthetic peptide aa 250-303 303 derived from human CDK4
Target NameCDK4
ConjugateFITC
Excitation Emission494nm 518nm
Other NamesCMM3; PSK-J3; Cyclin-dependent kinase 4; Cell division protein kinase 4; CDK4
Accession NoSwiss-Prot#P11802
NCBI Gene ID1019
Uniprot
P11802
Gene ID
1019;
Concentration1mg ml
Formulation0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
StorageShipped at 4˚C. Store at -20˚C for one year. Avoid repeated freeze/thaw cycles.
Application Details
Flow-Cyt=1:50-200 ICC=1:50-200 IF=1:50-200
Ser Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1) S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D CDK4 CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
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