Product Detail
Product NameAKT1 2 3 (Phospho-Ser472 Ser473 Ser474) Antibody HRP Conjugated
Host SpeciesRabbit
ClonalityPolyclonal
IsotypeIgG
PurificationPurified by Protein A.
ApplicationsWB IHC-P IHC-F
Species ReactivityHu Ms Rt
Immunogen DescKLH conjugated synthetic phosphopeptide aa 450-480 480 derived from human AKT1 around the phosphorylation site of Ser473
Target NameAKT1 2 3 (Ser472 Ser473 Ser474)
ConjugateHRP
Excitation EmissionN A
Other NamesAkt; RAC-alpha serine threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; RAC-PK-alpha; Akt1
Accession NoSwiss-Prot#P47196
NCBI Gene ID24185
Uniprot
P47196
Gene ID
24185;
Concentration1mg ml
Formulation0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
StorageShipped at 4˚C. Store at -20˚C for one year. Avoid repeated freeze/thaw cycles.
Application Details
WB=1:500-2000 IHC-P=1:50-200 IHC-F=1:50-200
AKT1-specific substrates have been recently identified, including palladin (PALLD), which phosphorylation modulates cytoskeletal organization and cell motility; prohibitin (PHB), playing an important role in cell metabolism and proliferation; and CDKN1A, for which phosphorylation at 'Thr-145' induces its release from CDK2 and cytoplasmic relocalization. These recent findings indicate that the AKT1 isoform has a more specific role in cell motility and proliferation. Phosphorylates CLK2 thereby controlling cell survival to ionizing radiation.
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15 business days.
