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Nemo-like kinase (Phospho-Thr298) Antibody HRP Conjugated#C00755H

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Product Detail

Product NameNemo-like kinase (Phospho-Thr298) Antibody HRP Conjugated

Host SpeciesRabbit

ClonalityPolyclonal

IsotypeIgG

PurificationPurified by Protein A.

ApplicationsWB,,IHC-P,IHC-F,ICC

Species ReactivityHu Ms Rt

Immunogen DescKLH conjugated synthetic phosphopeptide derived from human Nemo-like kinase around the phosphorylation site of Thr298

Target NameNemo-like kinase Thr298

ConjugateHRP

Excitation EmissionN A

Other NamesNemo-like kinase Thr298; Nemo-like kinase T298; p-Nemo-like kinase Thr298; p-Nemo-like kinase T298; DKFZp761G1211; FLJ21033; LAK1; Nemo like kinase; Nemo-like kinase; Nlk; NLK_HUMAN; Protein LAK1; Serine threonine protein kinase NLK; Serine threonine-protein kinase NLK.

Concentration1mg ml

Formulation0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

StorageShipped at 4˚C. Store at -20˚C for one year. Avoid repeated freeze/thaw cycles.

Application Details
WB=1:500-2000 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200
Nemo-like kinase (NLK) plays a role in cell fate determination and is required for differentiation of bone marrow stromal cells. It acts downstream of MAP3K7 and HIPK2 to negatively regulate the canonical Wnt beta-catenin signaling pathway and the phosphorylation and destruction of the MYB transcription factor. It may suppress a wide range of transcription factors by phosphorylation of the coactivator, CREBBP. (referenced from swissprot)

If you have published an article using product C00755H, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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