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Conjugated Chk2 (Phospho-Thr68) Rabbit mAb#C14265

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Product Detail

Product NameConjugated Chk2 (Phospho-Thr68) Rabbit mAb

Host SpeciesRecombinant Rabbit

ClonalityMonoclonal

PurificationAffinity-chromatography

Species ReactivityHuman

SpecificityPhospho-Chk2 (T68) Antibody detects endogenous levels of total Phospho-Chk2 (T68)

Immunogen DescA synthesized peptide derived from human Phospho-Chk2 (T68)

ConjugateAF Dye

Excitation EmissionAF350: 346nm/442nm;
AF405: 401nm/421nm;
AF488: 493nm/519nm;
AF555: 555nm/565nm;
AF594: 591nm/614nm;
AF647: 651nm/667nm;
AF680: 679nm/702nm;
AF750: 749nm/775nm;

Other NamesCHEK2; CHK2; Cds1; Chk2; EC 2.7.11.1; RAD53; kinase Chk2;

Accession NoUniprot:O96017

Uniprot O96017

Calculated MWPredicted band size: 61 kDa

Sdspage MWObserved band size: 65 kDa

FormulationLiquid in PBS, 0.5% BSA, 40% Glycerol, 0.02% Sodium Azide.

StorageStore at 4˚Cin dark for 6 months

Application Details
Suggested Dilution:;
AF350 conjugated: most applications: 1: 50 - 1: 250;
AF405 conjugated: most applications: 1: 50 - 1: 250;
AF488 conjugated: most applications: 1: 50 - 1: 250;
AF555 conjugated: most applications: 1: 50 - 1: 250;
AF594 conjugated: most applications: 1: 50 - 1: 250;
AF647 conjugated: most applications: 1: 50 - 1: 250;
AF680 conjugated: most applications: 1: 50 - 1: 250;
AF750 conjugated: most applications: 1: 50 - 1: 250;
Biotin conjugated: working with enzyme-conjugated streptavidin, most applications: 1: 50 - 1: 1,000;
These are known to be preferred sites for phosphorylation by ATM/ATR kinases. After DNA damage by ionizing radiation (IR), UV irradiation, or hydroxyurea treatment, Thr68 and other sites in this region become phosphorylated by ATM/ATR. The SQ/TQ cluster domain, therefore, seems to have a regulatory function.

If you have published an article using product C14265, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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