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Conjugated GRP78 BiP Rabbit mAb#C56023

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Product Detail

Product NameConjugated GRP78 BiP Rabbit mAb

Host SpeciesRecombinant Rabbit

ClonalityMonoclonal

PurificationAffinity-chromatography

Species ReactivityHuman Mouse Rat

SpecificityGRP78 BiP Antibody detects endogenous levels of total GRP78 BiP

Immunogen DescA synthesized peptide derived from human GRP78 BiP

ConjugateAF Dye

Excitation EmissionAF350: 346nm/442nm;
AF405: 401nm/421nm;
AF488: 493nm/519nm;
AF555: 555nm/565nm;
AF594: 591nm/614nm;
AF647: 651nm/667nm;
AF680: 679nm/702nm;
AF750: 749nm/775nm;

Other NamesGRP-78; GRP78; BIP; MIF2; HSPA5

Accession NoUniprot:P11021

Uniprot P11021

Calculated MW78kDa

FormulationLiquid in PBS, 0.5% BSA, 40% Glycerol, 0.02% Sodium Azide.

StorageStore at 4˚Cin dark for 6 months

Application Details
Suggested Dilution:;
AF350 conjugated: most applications: 1: 50 - 1: 250;
AF405 conjugated: most applications: 1: 50 - 1: 250;
AF488 conjugated: most applications: 1: 50 - 1: 250;
AF555 conjugated: most applications: 1: 50 - 1: 250;
AF594 conjugated: most applications: 1: 50 - 1: 250;
AF647 conjugated: most applications: 1: 50 - 1: 250;
AF680 conjugated: most applications: 1: 50 - 1: 250;
AF750 conjugated: most applications: 1: 50 - 1: 250;
Biotin conjugated: working with enzyme-conjugated streptavidin, most applications: 1: 50 - 1: 1,000;
When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) (PubMed 8020977) pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).

If you have published an article using product C56023, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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