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Conjugated HEC1 Rabbit mAb#C56209

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Product Detail

Product NameConjugated HEC1 Rabbit mAb

Host SpeciesRecombinant Rabbit

ClonalityMonoclonal

PurificationAffinity-chromatography

Species ReactivityHuman Mouse Rat

SpecificityHEC1 Antibody detects endogenous levels of total HEC1

Immunogen DescA synthesized peptide derived from human HEC1

ConjugateAF Dye

Excitation EmissionAF350: 346nm/442nm;
AF405: 401nm/421nm;
AF488: 493nm/519nm;
AF555: 555nm/565nm;
AF594: 591nm/614nm;
AF647: 651nm/667nm;
AF680: 679nm/702nm;
AF750: 749nm/775nm;

Other NamesHEC; HsHec1; hsNDC80; Kinetochore protein Hec1; Kinetochore-associated protein 2; KNTC2; ndc80; TID3;

Accession NoUniprot:O14777

Uniprot O14777

Calculated MW74kDa

FormulationLiquid in PBS, 0.5% BSA, 40% Glycerol, 0.02% Sodium Azide.

StorageStore at 4˚Cin dark for 6 months

Application Details
Suggested Dilution:;
AF350 conjugated: most applications: 1: 50 - 1: 250;
AF405 conjugated: most applications: 1: 50 - 1: 250;
AF488 conjugated: most applications: 1: 50 - 1: 250;
AF555 conjugated: most applications: 1: 50 - 1: 250;
AF594 conjugated: most applications: 1: 50 - 1: 250;
AF647 conjugated: most applications: 1: 50 - 1: 250;
AF680 conjugated: most applications: 1: 50 - 1: 250;
AF750 conjugated: most applications: 1: 50 - 1: 250;
Biotin conjugated: working with enzyme-conjugated streptavidin, most applications: 1: 50 - 1: 1,000;
Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.

If you have published an article using product C56209, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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