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Location： Home > ELISA Kits > Human PBEF/ Visfatin ELISA kit

Human PBEF/ Visfatin ELISA kit#EK0483

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Product Detail

Product NameHuman PBEF/ Visfatin ELISA kit

ApplicationsELISA

Species ReactivityHu

SpecificityNatural and recombinant Human PBEF/ Visfatin Ligand

Target NameHuman PBEF/ Visfatin

Application Details

Detect Range: 1-64ng/ml
Sensitivity: 0.5ng/mL
Sample Type: Cell culture supernatant, serum, plasma Sample Volume: 100 uL
Assay Time: 3 hours
Detection method: Sandwich

Representative standard curve for PBEF/Visfatin ELISA. PBEF/Visfatin was diluted in serial two-fold steps in Sample Diluent.

PBEF (preB cell colonyenhancing factor? also visfatin and nicotinamide phosphoribosyltransferase) is a 52 kDa member of the NAPRTase family of molecules. It functions both intracellularly and extracellularly, where it participates in NAD synthesis and insulin receptor activation, respectively. Human PBEF is 491 amino acids in length and contains no signal sequence. There is at least one alternative splice form that shows a 5 aa substitution for the Cterminal 128 amino acids (aa 364491). Over aa 27491, human PBEF shares 96%, 97%, and 96% aa identity with mouse, porcine, and canine PBEF, respectively.

If you have published an article using product EK0483, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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