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Rat Cytochrome P450, family 7, subfamily A, polypeptide 1 (CYP7A1) ELISA Kit#EK10942

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Product Detail

Product NameRat Cytochrome P450, family 7, subfamily A, polypeptide 1 (CYP7A1) ELISA Kit

ApplicationsELISA

Species ReactivityRat (Rattus norvegicus)

Other NamesCP7A; CYP7; MGC126826; MGC138389; cholesterol 7 alpha-monooxygenase|cholesterol 7-alpha-hydroxylase|cytochrome P450; subfamily VIIA (cholesterol 7 alpha-monooxygenase); polypeptide 1

Accession NoP51542

Uniprot P51542

Gene ID 100328551;

StorageThe stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37C for 4 and 7 days, and compare O.D.values of the kit kept at 37C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37C can be considered as 6 months at 2 - 8C, which means 7 days at 37C equaling 12 months at 2 - 8C).

Application Details
Detect Range:Request Information
Sensitivity:Request Information
Sample Type:Serum, Plasma, Other biological fluids
Sample Volume: 1-200 μL
Assay Time:1-4.5h
Detection wavelength:450 nm
Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate CYP7A1 in samples. An antibody specific for CYP7A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCYP7A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYP7A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYP7A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

If you have published an article using product EK10942, please notify us so that we can cite your literature.

et al,Deficiency of Epithelial PIEZO1 Alleviates Liver Steatosis Induced by High-Fat Diet in Mice. , (2025),
PMID: 39781454

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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