Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate AMBP in samples. An antibody specific for AMBP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAMBP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AMBP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AMBP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Alpha-1-microglobulin (A1M), also known as protein HC (for Heterogeneous Charge), is a low molecular weight protein component of plasma first discovered in pathological human urine. It is a member of the lipocalin superfamily. Although much is now known of its structure and properties, the function and physiological role of A1M remains unclear, although evidence suggests that it functions in the regulation of the immune system. A1M is known to exist in both a free form and complexed to other macromolecules: immunoglobulin A (IgA) in humans and alpha-1-inhibitor-3 in the rat. Free A1M is a monomeric protein composed of one 188 residue polypeptide and contains three cysteines, two of which (residues 75 and 173) form a conserved intra-molecular disulphide link.