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Mouse DNA mismatch repair protein Msh3(MSH3) ELISA Kit#EK20836

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Product Detail

Product NameMouse DNA mismatch repair protein Msh3(MSH3) ELISA Kit

ApplicationsELISA

Species ReactivityMouse

Application Details

Detect Range:0.156-10ng/mL
Sensitivity:less than 0.067ng/mL
Sample Type:Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Assay Time:1-4.5h
Detection wavelength:450 nm

 The microtiter plate provided in this kit has been pre-coated with an antibody specific to MSH3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to MSH3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MSH3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MSH3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Detection Method:sandwich

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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