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Sheep lmmunoglobulin A (lgA)ELISA Kit#EK21632

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Product Detail

Product NameSheep lmmunoglobulin A (lgA)ELISA Kit

ApplicationsELISA

Species ReactivitySheep

StorageThe stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37C for 4 and 7 days, and compare O.D.values of the kit kept at 37C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37C can be considered as 6 months at 2 - 8C, which means 7 days at 37C equaling 12 months at 2 - 8C).

Application Details

Detect Range:1.56 μg/mL - 100 μg/mL

Sensitivity:0.78 μg/mL

Sample Type:Serum, Plasma, Other biological fluids

Sample Volume: 1-200μL

Assay Time:1-4.5h

Detection wavelength:450 nm

The microtiter plate provided in this kit has been pre-coated with Sheep IgA. Samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated antibody preparation specific for Sheep IgA and incubated. Then substrate solutions are added to each well. Only those wells that contain Sheep IgA and enzyme-conjugated Sheep IgA will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ±2 nm.

If you have published an article using product EK21632, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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