Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate TTR in samples. An antibody specific for TTR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTTR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TTR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TTR bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:TTR was originally called prealbumin because it ran faster than albumins on electrophoresis gels.Prealbumin is produced by the choroid plexus, by pancreatic islet cells in the embryonic yolk sac, and by enterochromaffin cells in the gastrointestinal mucosa, but the liver is quantitatively the most important source.9 Liver production is maintained until late in liver disease. Hydration status does not affect prealbumin levels. A negative acute phase reactant, the prealbumin level will transiently decrease in the presence of inflammation and in the immediate postsurgical period. Serum levels also decline in patients with conditions associated with protein malnutrition, such as malignancy, cirrhosis, protein-losing enteropathy, and zinc deficiency.