Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate SERPINA5 in samples. An antibody specific for SERPINA5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySERPINA5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SERPINA5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPINA5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Protein C inhibitor (PCI, SERPINA5) is a serine protease inhibitor (serpin) which limits the expression of protein C.Suzuki et al. (1987) determined the sequence of cDNA for human protein C inhibitor (PCI). A high degree of homology of the deduced amino acid sequence to that of other known inhibitors clearly demonstrated that protein C inhibitor is a member of the superfamily of serine protease inhibitors including alpha-1-antichymotrypsin (AACT), alpha-1-antitrypsin (AAT; PI), antithrombin III (AT3), and angiotensinogen (AGT). Protein C inhibitor is sometimes referred to as plasminogen activator inhibitor-3 (PAI3) because it also inhibits plasminogen activators (Meijers and Chung, 1991). Plasminogen activator inhibitor-1 (PAI1) has structural similarities to PCI.