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Histone H4R3me1 Polyclonal Antibody#HW026

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Product Detail

Product NameHistone H4R3me1 Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

IsotypeIgG

PurificationAffinity purification

ApplicationsWB,IHC,IF

Species ReactivityHuman,Mouse,Rat

Immunogen TypePeptide

Immunogen DescA synthetic peptide of human MonoMethyl-Histone H4-R3

Target NameHistone H4

ConjugateUnconjugated

Other NamesHIST1H4B;H4/I;H4FI;histone H4;Histone H4;HIST1H4A;HIST2H4A;FO108;H4;H4/n;H4F2;H4FN;HIST2H4

Accession NoUniprot:P62805
GeneID:8366

Uniprot P62805

Gene ID 8366

Sdspage MW13kDa

Concentration1.0mg/ml

FormulationPBS with 0.02% sodium azide,50% glycerol,pH7.3.

StorageStore at -20˚C. Avoid freeze / thaw cycles.

Application Details
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using MonoMethyl-Histone H4-R3 antibody.
Immunohistochemistry of paraffin-embedded human colon using MonoMethyl-Histone H4-R3 antibody.
Immunofluorescence analysis of 293T cells using MonoMethyl-Histone H4-R3 antibody.
Immunohistochemistry of paraffin-embedded mouse brain using MonoMethyl-Histone H4-R3 antibody.
Immunohistochemistry of paraffin-embedded rat testis using MonoMethyl-Histone H4-R3 antibody.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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