Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Welcome Login Register My Cart(0,$0.00)
Location： Home > Methyl / Acetyl Antibodies > Histone H4R3me2s Polyclonal Antibody

Histone H4R3me2s Polyclonal Antibody#HW027

Datasheet PDF
Availability： Yes
Total Amount:$
ADD TO CART
Detail Pictures Description Publish(2) Other
Product Detail

Product NameHistone H4R3me2s Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits and were purified by antigen affinity-chromatography.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt

Immunogen TypePeptide

Immunogen DescA synthetic peptide corresponding to the amino terminus of histone H4 in which Arg3 is di-methylated.

Target NameHistone H4

ConjugateUnconjugated

Other NamesH4; H4/n; H4F2; H4FN; FO108; HIST2H4

Accession NoGene ID: 8290 Swiss Prot: Q16695

Uniprot Q16695

Gene ID 8290;

Sdspage MW11kDa

Concentration0.35mg/ml

FormulationBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

StorageStore at -20℃ or -80℃. Avoid freeze / thaw cycles.

Application Details

WB 1:500 - 1:2000

IHC 1:50 - 1:200

IF 1:50 - 1:200

Western blot analysis of extracts of HeLa cell line and H4 protein expressed in E.coli., using H4R3me2s antibody.
Dot-blot analysis of all sorts of methylation peptidesusing H4R3me2s antibody.
Immunofluorescence analysis of 293T cell using H4R3me2s antibody. Blue: DAPI for nuclear staining.

 Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.

If you have published an article using product HW027, please notify us so that we can cite your literature.

et al,Arginine methyltransferase inhibitor?1 inhibits sarcoma viability in vitro and in vivo.In Oncol Lett. On 2018 Aug by Zhang B, Chen X et al..PMID: 30008914 , (2018),
PMID: 30008914
et al,Arginine methyltransferase inhibitor 1 inhibits gastric cancer by downregulating eIF4E and targeting PRMT5.In Toxicol Appl Pharmacol on 2017 Dec by Baolai Zhang , Su Zhang,et al..PMID: 28987382 , (2017),
PMID: 28987382

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

QUICK NAV

New Products

Cell Signal Transduction

Cancer

Stem Cell

Neuroscience

Help and Support

Contact Us

Terms

Privacy Policy

support

Distributors