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Histone H3K9me3 Polyclonal Antibody#HW029

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Product Detail

Product NameHistone H3K9me3 Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

IsotypeIgG

PurificationAffinity purification

ApplicationsWB,IHC,IF

Species ReactivityHuman,Mouse,Rat

Immunogen TypePeptide

Immunogen DescA synthetic methylated peptide of human histone H3

Target NameHistone H3

ConjugateUnconjugated

Other NamesH3.4;H3/g;H3FT;H3t;HIST3H3;Histone H3;HIST1H3A

Accession NoUniprot:Q16695
GeneID:8290

Uniprot Q16695

Gene ID 8290

Sdspage MW17KDa

Concentration1.0mg/ml

FormulationPBS with 0.02% sodium azide,50% glycerol,pH7.3.

StorageStore at -20˚C. Avoid freeze / thaw cycles.

Application Details
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K9 antibody.
Immunohistochemistry of paraffin-embedded mouse testis using TriMethyl-Histone H3-K9 antibody.
Immunohistochemistry of paraffin-embedded rat testis using TriMethyl-Histone H3-K9 antibody.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

If you have published an article using product HW029, please notify us so that we can cite your literature.

et al,ZMYND10 downregulates cyclins B1 and D1 to arrest cell cycle by trimethylating lysine 9 on histone 3 , (2021),
PMID:
et al,AMBRA1 drives gastric cancer progression through regulation of tumor plasticity , (2024),
PMID:

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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