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Histone H3K14me3 Polyclonal Antibody#HW036

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Product NameHistone H3K14me3 Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits and were purified by antigen affinity-chromatography.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt

Immunogen TypePeptide

Immunogen DescA synthetic peptide of human H3K14me3

Target NameHistone H3

ConjugateUnconjugated

Other NamesH3t; H3.4; H3/g; H3FT;

Accession NoGene ID: 8290 Swiss Prot: Q16695

Uniprot Q16695

Gene ID 8290;

Sdspage MW15kDa

Concentration1.0mg/ml

FormulationBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

StorageStore at -20℃ or -80℃. Avoid freeze / thaw cycles.

Application Details

WB 1:500 - 1:2000

IHC 1:50- 1:200

IF 1:20- 1:50

Western blot analysis of extracts of HeLa and H3 protein, using H3K14me3 antibody.

 Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

If you have published an article using product HW036, please notify us so that we can cite your literature.

et al,A transient ischemic environment induces reversible compaction of chromatin.In Genome Biol on 2015 Nov 5 by Ina Kirmes, Aleksander Szczurek et al..PMID:26541514 , (2015),
PMID: 26541514

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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