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Histone H3(mono-methyl-K79) Mouse Monoclonal Antibody (1E10)#HW044

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Product NameHistone H3(mono-methyl-K79) Mouse Monoclonal Antibody (1E10)

Clone No.1E10

Host SpeciesMouse

ClonalityMonoclonal

PurificationAffinity purification using immunogen.

ApplicationsWB

Species ReactivityHu Rt Ms

SpecificityAntibody detects endogenous Histone H3 (mono-methyl-K79) protein.

Immunogen Typepeptide

Immunogen DescA synthetic Mono-methylated peptide corresponding to residues surrounding K79 of human histone H3.

Target NameHistone H3(mono-methyl-K79)

Accession NoSwiss-Prot#:P68431

Uniprot P68431

Gene ID 8350;8351;8352;8353;8354;8355;8356;8357;8358;8968;

Sdspage MW17kd

Concentration1.0mg/ml

FormulationMouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C

Application Details
Western blotting: 1:500~1:2000
Western blot analysis of Hela cells, using #HW044 diluted at 1) 1:1,000, 2) 1:3,000.
Western blot analysis of 1) Rat Testis tissue, 2) Raw264.7, using #HW044 diluted at 1:2000.
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.

If you have published an article using product HW044, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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