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NFκB-p65 (Acetyl-Lys221) Polyclonal Antibody#HW149

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Total Amount:$
Product Detail

Product NameNFκB-p65 (Acetyl-Lys221) Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ApplicationsWB ELISA

Species ReactivityHu Ms Rt

SpecificityAcetyl-NFκB-p65 (K221) Polyclonal Antibody detects endogenous levels of NFκB-p65 protein only when acetylation at K221.

Immunogen DescSynthesized peptide derived from the Internal region of human NFκB-p65 around the acetylation site of K221.

Target NameNFκB-p65

ConjugateUnconjugated

Other NamesRELA; NFKB3; Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3

Accession NoSwiss-Prot: Q04206
NCBI Gene ID: 5970

Uniprot Q04206

Gene ID 5970;

Sdspage MW65kd

Concentration1mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

StorageStore at -20˚C/1 year

Target Specieshuman

Application Details

Western blotting: 1/500 - 1/2000
ELISA: 1/10000
Not yet tested in other applications
 

Western Blot analysis of HEPG2-UV cells using Acetyl-NFκB-p65 (K221) Polyclonal Antibody
Western Blot analysis of HepG2-UVmouse brain cells using Acetyl-NFκB-p65 (K221) Polyclonal Antibody

If you have published an article using product HW149, please notify us so that we can cite your literature.

et al,Ghrelin Represses Thymic Stromal Lymphopoietin Gene Expression through Activation of Glucocorticoid Receptor and Protein Kinase C Delta in Inflamed Skin Keratinocytes. In Int J Mol Sci on 2022 Apr 2 by Hayan Jeong, Hyo-Jin Chong, et al..PMID: 35409338 , (2022),
PMID: 35409338

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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