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Histone H3 (Tri-Methyl-K5) Polyclonal Antibody#HW172

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Product Detail

Product NameHistone H3 (Tri-Methyl-K5) Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityThis antibody detects endogenous levels of Histone H3 (Tri-Methyl-K5), It doesn't reacte with total or Di-Methy or Mono-Methyl protein.

Immunogen DescSynthesized Tri-Methyl peptide derived from human Histone H3. at AA range: K5

Target NameHistone H3 (Tri-Methyl-K5)

ConjugateUnconjugated

Other NamesHistone H3

Accession NoSwiss-Prot:P68431/Q71DI3/P84243/Q6NXT2
NCBI Gene ID:8350

Uniprot P68431

Gene ID 8350;8351;8352;8353;8354;8355;8356;8357;8358;8968;

Calculated MW17 kDa

Concentration1 mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

StorageStore at -20˚C

Application Details

Western blotting: 1:500-1:2000

Western Blot analysis of hela cells using primary antibody diluted at 1:1000(˚C overnight). Secondary antibody£ºGoat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25¡ãC, 1 hour)

If you have published an article using product HW172, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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