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p53 (Acetyl-K120) Polyclonal Antibody#HW186

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Product Namep53 (Acetyl-K120) Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityThis antibody detects endogenous levels of p53 (Acetyl-K120), It doesn't reacte with total protein.

Immunogen Typeprotein

Immunogen DescSynthesized Acetyl peptide derived from human p53. at AA range: K120

Target Namep53 (Acetyl-K120)

ConjugateUnconjugated

Other NamesCellular tumor antigen p53 (Antigen NY-CO-13) (Phosphoprotein p53) (Tumor suppressor p53)

Accession NoSwiss-Prot:P04637
NCBI Gene ID:7157

Uniprot P04637

Gene ID 7157;

Calculated MW53 kDa

Concentration1 mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

StorageStore at -20˚C

Application Details
Western blotting: 1£º500-1£º2000
Western Blot analysis of 1,mouse-liver 2,hela 3,mouse-brain cells using primary antibody diluted at 1:1000(˚C overnight). Secondary antibody£ºGoat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25¡ãC, 1 hour)

If you have published an article using product HW186, please notify us so that we can cite your literature.

et al,A GYS2/p53 Negative Feedback Loop Restricts Tumor Growth in HBV-Related Hepatocellular CarcInoma. In Cancer Res on 2019 Feb 1 by Chen SL, Zhang CZ, et al..PMID:30584071 , (2019),
PMID: 30584071
et al,SIRT1 inhibitors mitigate radiation-induced GI syndrome by enhancing intestinal-stem-cell survival. In Cancer Lett on 2021 Mar 31 by Guoxiang Fu, Shengzhi Chen, et al..PMID:33359449 , (2021),
PMID: 33359449

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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