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PARP-1 (Acetyl-K521) Polyclonal Antibody#HW188

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Product Detail

Product NamePARP-1 (Acetyl-K521) Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityThis antibody detects endogenous levels of PARP-1 (Acetyl-K521), It doesn't reacte with total protein.

Immunogen Typeprotein

Immunogen DescSynthesized Acetyl peptide derived from human PARP-1. at AA range: K521

Target NamePARP-1 (Acetyl-K521)

ConjugateUnconjugated

Other NamesPoly [ADP-ribose] polymerase 1 (PARP-1) (EC 2.4.2.30) (ADP-ribosyltransferase diphtheria toxin-like 1) (ARTD1) (NAD(+) ADP-ribosyltransferase 1) (ADPRT 1) (Poly[ADP-ribose] synthase 1)

Accession NoSwiss-Prot:P09874
NCBI Gene ID:142

Uniprot P09874

Gene ID 142;

Calculated MW113 kDa

Concentration1 mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

StorageStore at -20˚C

Application Details
Western blotting: 1£º500-1£º2000
Western Blot analysis of 1,mouse-heart 3,mouse-brain cells using primary antibody diluted at 1:1000(˚C overnight). Secondary antibody£ºGoat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25¡ãC, 1 hour)
Western Blot analysis of 1,293t 2,mouse-brain cells using primary antibody diluted at 1:1000(˚C overnight). Secondary antibody£ºGoat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25¡ãC, 1 hour)

If you have published an article using product HW188, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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