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SCAP (Phospho-Tyr300) Antibody#SAB626

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Product Detail

Product NameSCAP (Phospho-Tyr300) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody was purified from rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

ApplicationsWB

Species ReactivityHuman,Mouse

SpecificitySCAP(Phospho-Tyr300) Antibody detects endogenous levels of SCAP only when phosphorylated at Tyr300.

Immunogen DescA synthesized peptide derived from human SCAP around the phosphorylation site of Tyr300.

ConjugateUnconjugated

Other NamesSCAP,SREBP cleavage-activating protein,KIAA0199,PSEC0227

Uniprot Q12770

Gene ID 22937

Sdspage MW139kDa

Concentration1 mg/ml

FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl,0.02% sodium azide and 50% glycerol.

StorageStore at --20˚C/1 year

Application Details
Western Blot: 1/500 - 1/2000
Escort protein required for cholesterol as well as lipid homeostasis (By similarity). Regulates export of the SCAP-SREBP complex from the endoplasmic reticulum to the Golgi upon low cholesterol, thereby regulating the processing of sterol regulatory element-binding proteins (SREBPs) SREBF1/SREBP1 and SREBF2/SREBP2 (By similarity). At high sterol concentrations, formation of a ternary complex with INSIG (INSIG1 or INSIG2) leads to mask the ER export signal in SCAP, promoting retention of the complex in the endoplasmic reticulum (By similarity).

If you have published an article using product SAB626, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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